Movie S3

Time-lapse movie of mitotic HeLa cells expressing EGFP-fused ΔPro-all importin β. Mitotic HeLa cells expressing EGFP-fused ΔPro-all mutant importin β and mPlum-histone H3 were observed under a confocal laser scanning microscope. Images were taken every 2 min. The nuclear membrane did not breakdown throughout the observation period (50 min in total).