Movie S3

Time-lapse movie of mitotic HeLa cells expressing EGFP-fused ΔPro-all importin β. Mitotic HeLa cells expressing EGFP-fused ΔPro-all mutant importin β and mPlum-histone H3 were observed under a confocal laser scanning microscope. Images were taken every 2 min. The nuclear membrane did not breakdown throughout the observation period (50 min in total).

Prolines in the α-helix confer the structural flexibility and functional integrity of importin-β

Masahiro Kumeta, Hide A. Konishi, Wanzhen Zhang, Sayuri Sakagami, and Shige H. Yoshimura

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.206326