Movie S3

The dynamic nature of neural rosette lumens.
Human neural rosettes were harvested at 10-day differentiation and were transferred to a new Matrigel and fibronectin-coated plate. Spread-out neural rosettes were subjected to time-lapse imaging using BioStation CT (Nikon Corporation, Japan). Image acquisition timing was set to every 30 min per picture for 3 days. The movie presented here was a part of a full-length film. The movie shows that the lumens of neural rosettes are constantly reshaping or re-structuring when rosettes are growing and thickening, indicating the dynamic nature of the lumens.

Calcium signaling mediates five types of cell morphological changes to form neural rosettes

Hana Hříbková, Marta Grabiec, Dobromila Klemová, Iva Slaninová, and Yuh-Man Sun

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.206896