Movie S3

The dynamic nature of neural rosette lumens.
Human neural rosettes were harvested at 10-day differentiation and were transferred to a new Matrigel and fibronectin-coated plate. Spread-out neural rosettes were subjected to time-lapse imaging using BioStation CT (Nikon Corporation, Japan). Image acquisition timing was set to every 30 min per picture for 3 days. The movie presented here was a part of a full-length film. The movie shows that the lumens of neural rosettes are constantly reshaping or re-structuring when rosettes are growing and thickening, indicating the dynamic nature of the lumens.