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Video S1

Related to Figure 1. IL6Rchi responds to extracellular IL6. The IL6 detector cell line expressing IL6Rchi and the Ca2+ sensor RCaMP1.07 responded to 10 ng/mL bolus addition of IL6 to the media. The detector cells show an oscillation in fluorescence intensity, indicative of a Ca2+ signal. Scale bar is 100 μm. Experiment was repeated at least 3 times.

Engineering mammalian cells to seek senescence-associated secretory phenotypes

Anam Qudrat, Janice Wong, and Kevin Truong

J Cell Sci 2017. 130:3116-3123; doi: 10.1242/jcs.206979