A representative time-lapse video of Prosβ5-RFP particles trafficking in axonal tracts of a control third-instar larvae. The video was acquired using a 100X objective at the temporal resolution of 1.8 Hz (500 ms exposure and an average time lapse of 570 ms). The video is shown at 10X the acquired speed. (Genotype: C380-Gal4/+;UAS-prosβ5-RFP/+; UAS-prosβ5-RFP/+)