To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 2. EB3-GFP localised to the axon initial segment and cell body (comets) of E18 15DIV cortical neuron, also expressing shRNA targeting EFA6. The apparent break in the axon is a section of axon out of the plane of focus. EFA6 regulates selective polarised transport and axon regeneration from the axon initial segment Richard Eva, Hiroaki Koseki, Venkateswarlu Kanamarlapudi, and James W. Fawcett J Cell Sci 2017. 130:3663-3675; doi: 10.1242/jcs.207423 Download Original Video