Movie S1

Three Dimensional Images of YFP-RabA4b-decorated Vesicles in the Pollen Tube Before and After Subtraction of Weak Signals
Three-dimensional projection of stacked images was generated by ImageJ using the “BrightestPoint” projection method with the incremeonft rotation angle set at 2. Interpolation was avoided. The left and right panels represent the three-dimensional images before and after the subtraction of the average fluorescence in the yellow boxed region in the shank as described in Fig. 5C. Bar = 5 μm.

ADF10 shapes the overall organization of apical actin filaments by promoting their turnover and ordering in pollen tubes

Yuxiang Jiang, Juan Wang, Yurong Xie, Naizhi Chen, and Shanjin Huang

J Cell Sci 2017. 130:3988-4001; doi: 10.1242/jcs.207738