Video 2
Typical TIRFM recording of Ca2+ puffs from a field of HEK293 cell loadedwith ci-IP3 and stimulated with a UV-flash (40 ms) after 20 s. The video, showing unprocessed images, was captured and replayed at 25 fps. Scale bar = 10 μm.
Endogenous signalling pathways and caged IP3 evoke Ca2+ puffs at the same abundant immobile intracellular sites
J Cell Sci 2017. 130:3728-3739; doi: 10.1242/jcs.208520