Mosaic deletion of both NMIIA and IIB induces mammary organoid overgrowth in basal medium conditions. Representative DIC time-lapse movies of control (wild-type) and NMIIA,B-null organoid treated with Ad-Cre cultured in 3D Matrigel under basal medium conditions (No GF). Normal epithelium (left) maintained same size. NMIIA,B-null organoid (right) resulted in epithelium tissue overgrowth. Frames were collected every 20 minutes for 100 hours (displayed at 13 frames/s) using a Cell Observer system with an AxioObserver Z1 and an AxioCam MRM camera (Carl Zeiss). Bars, 50 μm.