Movie 2

NMIIA and NMIIB double deletion induces cell division of both wild-type and NMIIA,B-null cells in mosaic organoids. Representative confocal time-lapse movie of a mosaic NMIIA,B-null organoid (2D slice) showing cell divisions in both Cre- (red) and Cre+ (green) cells under basal medium conditions (No GF). Frames were collected every 20min for 33h (displayed at 15 frames/s) using a spinning-disk confocal microscope (Solamere Technology Group Inc.) with an LD C-Apochromat 40×/1.1 W Korr objective lens (Carl Zeiss). Bars, 20 μm.

Mosaic loss of non-muscle myosin IIA and IIB is sufficient to induce mammary epithelial proliferation

Kim-Vy Nguyen-Ngoc, Vanesa L. Silvestri, Dan Georgess, Amanda N. Fairchild, and Andrew J. Ewald

J Cell Sci 2017. 130:3213-3221; doi: 10.1242/jcs.208546