Supplementary Video 1

Dissociation of cdc10(G100E)-GFP from the bud-neck into cytoplasm during starvation. cdc10(G100E)-GFP cells expressing mCh-Atg8 were grown in SD-Ura medium at 22°C and the logarithmically growing cells were then transferred into starvation media and incubated at 22°C and 37°C. Immediately after the transfer into starvation media, 1 OD cells were collected as 0 h time point and were mounted on agarose pads (made in SD-N medium). Time-lapse was carried out for 30 min with an interval of 2 min at 22°C. Only GFP channel is shown. Scale bar 5 μm.