Supplementary Video 2

Cdc10-GFP is shown as representative of the three septins. Dynamic interaction of Cdc10-GFP with mCh-Atg8 is shown here. Cells were grown in SD-Ura medium at 30°C and the logarithmically growing cells were then transferred into starvation media. After 4 h incubation in starvation media, 1 OD cells were collected and were mounted on agarose pads (made in SD-N medium). Time-lapse was carried out for 20 min with an interval of 4 min at RT Scale bar 2 μm.

Septins are involved at the early stages of macroautophagy in S. cerevisiae

Gaurav Barve, Shreyas Sridhar, Amol Aher, Mayurbhai H. Sahani, Sarika Chinchwadkar, Sunaina Singh, Lakshmeesha K. N., Michael A. McMurray, and Ravi Manjithaya

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.209098