Supplementary Video 3

BiFC study that shows Cdc10 transiently interact with Atg9 at PAS (Ape1-RFP). Cells expressing Cdc10-Vc, Atg9-Vn and Ape1-RFP were grown in SD-his medium at 30°C and the logarithmically growing cells were then transferred into starvation media. After 5 h incubation in starvation media, 1 OD cells were collected and were mounted on agarose pads (made in SD-N medium). Time-lapse was carried out for 100 min with an interval of 10 min at RT. Scale bar 2 μm.

Septins are involved at the early stages of macroautophagy in S. cerevisiae

Gaurav Barve, Shreyas Sridhar, Amol Aher, Mayurbhai H. Sahani, Sarika Chinchwadkar, Sunaina Singh, Lakshmeesha K. N., Michael A. McMurray, and Ravi Manjithaya

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.209098