To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 1 FIB/SEM reveals 3D organisation of ER and rBCVs. FIB/SEM tomogram of a HeLa cell 24 h.p.i. depicting an single rBCV. The ER cisternae in proximity of the rBCV (arrows) are connected to the vacuole through a thin membrane extension (arrow head). 3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum Jaroslaw Sedzicki, Therese Tschon, Shyan Huey Low, Kevin Willemart, Kenneth N. Goldie, Jean-Jacques Letesson, Henning Stahlberg, and Christoph Dehio J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.210799 Download Original Video