Movie S2

Adjacent Sister Placement
Confocal LSM imaging of an induced wild-type H2B-GFP organoid showing manual tracking of a mitotic cell and its progeny. Daughters were tracked manually using Imaris. In this example, both daughter cells ‘re-insert’ into the epithelium as neighbours.

Interkinetic nuclear migration and basal tethering facilitates post-mitotic daughter separation in intestinal organoids

Thomas D. Carroll, Alistair J. Langlands, James M. Osborne, Ian P. Newton, Paul L. Appleton, and Inke Näthke

J Cell Sci 2017. 130:3862-3877; doi: 10.1242/jcs.211656