Movie S3

Post-mitotic Separation
Confocal LSM imaging of an induced wild-type H2B-GFP organoid showing manual tracking of a mitotic cell and its progeny. Daughters were tracked manually using Imaris. In this example, the ‘blue’ daughter cell is displaced from its sister.

Interkinetic nuclear migration and basal tethering facilitates post-mitotic daughter separation in intestinal organoids

Thomas D. Carroll, Alistair J. Langlands, James M. Osborne, Ian P. Newton, Paul L. Appleton, and Inke Näthke

J Cell Sci 2017. 130:3862-3877; doi: 10.1242/jcs.211656