Movie S4

4D Visualisation of post-mitotic separation
Confocal LSM imaging of an induced wild-type H2B-GFP organoid showing manual tracking of a mitotic cell and its progeny undergoing post-mitotic separation. Surface rendering was performed to highlight the mother (cyan), sisters (blue/red) and neighbour cells (magenta). The respective timelapse is shown in the top panels and a 3D rotation around the timepoints encompassing interphase, INM, cytokinesis and after separation are displayed in the bottom panels.

Interkinetic nuclear migration and basal tethering facilitates post-mitotic daughter separation in intestinal organoids

Thomas D. Carroll, Alistair J. Langlands, James M. Osborne, Ian P. Newton, Paul L. Appleton, and Inke Näthke

J Cell Sci 2017. 130:3862-3877; doi: 10.1242/jcs.211656