Movie 11

Time-lapse image acquisition, T. obliquus dividing in a microfluidic device. Laser scanning confocal microscopy was used to acquire chloroplast fluorescence. Chloroplast morphology was used as a proxy for cell shape and location. In this acquisition, three cells (labeled 1-3, at time 0) underwent cell mass rotation within the mother cell wall (described in Fig. 3). Four daughters emerged from the central cell at later time points (230-250 min, described in Fig. S1). Release of daughter cells is evident as an unfolding of the multicellular coenobium containing one fluorescent chloroplast per cell.