To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 1 relating to Figure 2 RUSH trafficking in WT cells. WT cells transfected with Str-Kdel/ManIISBP-EGFP were treated with biotin at T0 and imaged live as a single plane at 4 frames per minute. Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression Nicola L. Stevenson, Dylan J. M. Bergen, Roderick E. H. Skinner, Erika Kague, Elizabeth Martin-Silverstone, Kate A. Robson Brown, Chrissy L. Hammond, and David J. Stephens J Cell Sci 2017. 130:4132-4143; doi: 10.1242/jcs.212308 Download Original Video