Movie 2 relating to Figure 2
RUSH trafficking in KO cells. Giantin KO cells transfected with Str-Kdel/ManII-SBP-EGFP were treated with biotin at T0 and imaged live as a single plane at 4 frames per minute.
Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression
J Cell Sci 2017. 130:4132-4143; doi: 10.1242/jcs.212308