Movie 2

Initial actin and Tpm3.1 filaments simultaneously assemble around fused secretory granules in mouse acinar cells in vivo. Related to Figures 4A and S3.
Intravital confocal imaging of actin filament assembly during secretory granule exocytosis in submandibular salivary gland acinar cells in Tpm3.1-NG KI x Lifeact-RFP mouse. Single exocytosis event acquired at higher temporal resolution (241 ms image frame intervals) is shown. Tpm3.1-NG (green) and Lifeact-RFP (red) appear to be recruited simultaneously around the granule. Time zero is set to one frame prior to first appearance of Lifeact-RFP signal on the secretory granule. Playback was set to 60 frames per sec (approximately 16x real time). Time is displayed in seconds.milliseconds. Scale bar = 5 μm.