Movie 3

Myosin IIA-GFP recruitment follows Initial actin filament assembly around fused secretory granules in mouse acinar cells in vivo. Related to Figures 4B and S4.
Intravital confocal imaging of actin filament assembly during secretory granule exocytosis in submandibular salivary gland acinar cells in Myosin IIA-GFP KI x Lifeact-RFP mouse. Single exocytosis event acquired at higher temporal resolution (241 ms image frame intervals) is shown. The initial recruitment of Myosin IIA-GFP (green) around the granule seems to lag a few seconds behind the first appearance of Lifeact-RFP (red). Playback was set to 60 frames per sec (approximately 16x real time). Time is displayed in seconds.milliseconds. Scale bar = 5 μm.