Supplementary Movie 4

Animation of cellular staining of Naegleria gruberi cells using confocal microscopy. 3D-stacked animation displaying the localisation of COPB (green) and DAPI (blue) after incubation with 10 nM of Brefeldin A for three hours in a 3D rendering of 32 individual, 0.29 μm thick sections, overlapping with a final representative thickness of 9.27 μm, displayed in Fig. 4D.