Supplementary Movie 5

Animation of cellular staining of Naegleria gruberi cells using confocal microscopy. 3D-stacked animation displaying the localisation of COPB (green) and DAPI (blue) after incubation with 1 μM of Brefeldin A for three hours in a 3D rendering of 26 individual, 0.29 μm thick sections, overlapping with a final representative thickness of 7.53 μm, displayed in Fig. 4E.