Movie S1

Membrane-tethering of MEK-2 results in a cell polarity defect during spore germination. The strain used is 286 (mek-2::hph;his3::Ptef-1-mek-2-gfp-caax). Spores were incubated for 90 minutes on solid minimal medium. Time is indicated in minutes. Scale bar: 5 μm.