Supplemental Movie 1
Endo/lysosome dynamics of DMSO-treated cells at high-frame rate imaging using spinning disc confocal microscopy. RAW macrophages were pre-labelled with Alexa546-conjugated dextran, followed by live-cell imaging using spinning disc confocal microscopy after the addition of DMSO. Imaging was done by acquiring 6 planes along the z-axis every 10 s for 30 min. Refer to Supplemental Figure S6 for select stills. Scale and time are indicated.
Lysosome enlargement during inhibition of the lipid kinase PIKfyve proceeds through lysosome coalescence
J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.213587