Endo/lysosome dynamics of DMSO-treated cells at high-frame rate imaging using spinning disc confocal microscopy. RAW macrophages were pre-labelled with Alexa546-conjugated dextran, followed by live-cell imaging using spinning disc confocal microscopy after the addition of DMSO. Imaging was done by acquiring 6 planes along the z-axis every 10 s for 30 min. Refer to Supplemental Figure S6 for select stills. Scale and time are indicated.
Christopher H. Choy, Golam Saffi, Matthew A. Gray, Callen Wallace, Roya M. Dayam, Zhen-Yi A. Ou, Guy Lenk, Rosa Puertollano, Simon C. Watkins, and Roberto J. Botelho
J Cell Sci
2018. 131:None-None; doi: 10.1242/jcs.213587