Supplemental Movie 1

Endo/lysosome dynamics of DMSO-treated cells at high-frame rate imaging using spinning disc confocal microscopy. RAW macrophages were pre-labelled with Alexa546-conjugated dextran, followed by live-cell imaging using spinning disc confocal microscopy after the addition of DMSO. Imaging was done by acquiring 6 planes along the z-axis every 10 s for 30 min. Refer to Supplemental Figure S6 for select stills. Scale and time are indicated.

Lysosome enlargement during inhibition of the lipid kinase PIKfyve proceeds through lysosome coalescence

Christopher H. Choy, Golam Saffi, Matthew A. Gray, Callen Wallace, Roya M. Dayam, Zhen-Yi A. Ou, Guy Lenk, Rosa Puertollano, Simon C. Watkins, and Roberto J. Botelho

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.213587