Supplemental Movie 2

Endo/lysosome dynamics of apilimod-treated cells at high-frame rate imaging using spinning disc confocal microscopy. RAW macrophages were pre-labelled with Alexa⁵⁴⁶-conjugated dextran as before, followed by live-cell imaging using spinning disc confocal microscopy after the addition of 20 nM apilimod. Imaging was done by acquiring 6 planes along the z-axis once every 10 s for 30 min after apilimod addition. Refer to Supplemental Figure S6 for select stills. Vacuolation was not observed in the field under observation. Scale and time are indicated.