Supplemental Movie 2

Endo/lysosome dynamics of apilimod-treated cells at high-frame rate imaging using spinning disc confocal microscopy. RAW macrophages were pre-labelled with Alexa546-conjugated dextran as before, followed by live-cell imaging using spinning disc confocal microscopy after the addition of 20 nM apilimod. Imaging was done by acquiring 6 planes along the z-axis once every 10 s for 30 min after apilimod addition. Refer to Supplemental Figure S6 for select stills. Vacuolation was not observed in the field under observation. Scale and time are indicated.

Lysosome enlargement during inhibition of the lipid kinase PIKfyve proceeds through lysosome coalescence

Christopher H. Choy, Golam Saffi, Matthew A. Gray, Callen Wallace, Roya M. Dayam, Zhen-Yi A. Ou, Guy Lenk, Rosa Puertollano, Simon C. Watkins, and Roberto J. Botelho

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.213587