Supplemental Movie 3

Endo/lysosome dynamics of DMSO-treated cells at low-frame rate imaging using spinning disc confocal microscopy. RAW macrophages were pre-labelled with Alexa⁵⁴⁶-conjugated dextran as before, followed by live-cell imaging using spinning disc confocal microscopy after the addition of DMSO. Imaging was done at a single-plane once every 2 min for 60 min. Refer to Figure 8A for select stills. Scale and time are indicated.