Supplemental Movie 4

Endo/lysosome dynamics of apilimod-treated cells at low-frame rate imaging using spinning disc confocal microscopy. RAW macrophages were pre-labelled with Alexa⁵⁴⁶-conjugated dextran as before, followed by live-cell imaging using spinning disc confocal microscopy after the addition of 20 nM apilimod. Imaging was done at a single-plane once every 2 min for 60 min. Refer to Figure 8B for select stills. Vacuolation occurs during lower light exposure frequency. Scale and time are indicated.