Supplemental Movie 4

Endo/lysosome dynamics of apilimod-treated cells at low-frame rate imaging using spinning disc confocal microscopy. RAW macrophages were pre-labelled with Alexa546-conjugated dextran as before, followed by live-cell imaging using spinning disc confocal microscopy after the addition of 20 nM apilimod. Imaging was done at a single-plane once every 2 min for 60 min. Refer to Figure 8B for select stills. Vacuolation occurs during lower light exposure frequency. Scale and time are indicated.

Lysosome enlargement during inhibition of the lipid kinase PIKfyve proceeds through lysosome coalescence

Christopher H. Choy, Golam Saffi, Matthew A. Gray, Callen Wallace, Roya M. Dayam, Zhen-Yi A. Ou, Guy Lenk, Rosa Puertollano, Simon C. Watkins, and Roberto J. Botelho

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.213587