Supplemental Movie 6

Endo/lysosome dynamics of apilimod-treated cells at high-frame rate imaging using sweptfield confocal microscopy. RAW macrophages were pre-labelled with Alexa555-conjugated dextran, followed by live-cell imaging using sweptfield confocal microscopy after the addition of 20 nM apilimod. Imaging was done by acquiring 10 planes along the z-axis every 30 s for 2 h. The movie depicts a projection of all z-planes. Refer to Figure 8D for select stills. Scale and time are indicated.

Lysosome enlargement during inhibition of the lipid kinase PIKfyve proceeds through lysosome coalescence

Christopher H. Choy, Golam Saffi, Matthew A. Gray, Callen Wallace, Roya M. Dayam, Zhen-Yi A. Ou, Guy Lenk, Rosa Puertollano, Simon C. Watkins, and Roberto J. Botelho

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.213587