E-Cad dynamics during wound closure in wild type and Grh KD. Notum epithelia expressing E-Cad::GFP, labeling E-Cad. Wild type shown on left and Grh KD shown on the right. Red arrowheads show decrease of E-Cad cortical levels on the right. Images were acquired using a spinning disk imaging system (Revolution XD; Andor Technology). Frames were taken every 1 min. Scale bar: 20 μm.