Video S10

Actin and myosin dynamics during cortical collapse in Grh KD. Notum epithelia expressing Sqh::GFP, and mCherry::Moesin under the control of sr-Gal4 driver, labeling myosin and F-actin, respectively. Myosin is shown on the left (green in merge), F-actin in the middle (magenta in merge) and merged image on the right. Zoomed areas represent portions limited by dashed white square, showing decrease in cortical myosin (left image) is followed by emerging filopodia (middle image). Movie starts 60 mpw. Images were acquired using a spinning disk imaging system (Revolution XD; Andor Technology). Frames were taken every 1 min. Scale bar: 20 μm and 10 μm in zoomed areas.