Supplemental Movie 3
Dynamics of eGFP-tagged FGFR wild type and the activating mutant N550K intracellular vesicles in mCherry- Rab6a (Golgi) expressing Ishikawa cells. Spinning disc confocal microscopy time-lapse sequence cells co-expressing either, top panel: FGFRWT-eGFP (green) and Rab6a-mCherry (magenta) or bottom panel: FGFR-N550K- eGFP (green) and mCherry-Rab6a (magenta). eGFP vesicles are highly dynamic in both WT and N550K and move independently of Golgi structures (mCherry-Rab6a). Images were acquired every 1 second for 2 minutes. The video plays at 10 frames s-1 and is thus accelerated 10 times.