Supplemental Movie 3

Dynamics of eGFP-tagged FGFR wild type and the activating mutant N550K intracellular vesicles in mCherry- Rab6a (Golgi) expressing Ishikawa cells. Spinning disc confocal microscopy time-lapse sequence cells co-expressing either, top panel: FGFRWT-eGFP (green) and Rab6a-mCherry (magenta) or bottom panel: FGFR-N550K- eGFP (green) and mCherry-Rab6a (magenta). eGFP vesicles are highly dynamic in both WT and N550K and move independently of Golgi structures (mCherry-Rab6a). Images were acquired every 1 second for 2 minutes. The video plays at 10 frames s-1 and is thus accelerated 10 times.

FGFR2-activating mutations disrupt cell polarity to potentiate migration and invasion in endometrial cancer cell models

Samantha J. Stehbens, Robert J. Ju, Mark N. Adams, Samuel R. Perry, Nikolas K. Haass, David M. Bryant, and Pamela M. Pollock

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.213678