Supplemental Movie 4

Dynamics of eGFP-tagged FGFR wild type and the activating mutant Y376C intracellular vesicles in Sec61-mCherry (ER) expressing Ishikawa cells. Spinning disc confocal microscopy time-lapse sequence cells co-expressing either, top panel: FGFR-WTeGFP (green) and Sec-61-mCherry (magenta) and corresponding grey scale image below or bottom panel: FGFR-Y376C- eGFP (green) and Sec-61-mCherry (magenta) and corresponding grey scale image below. eGFP vesicles are highly dynamic in both WT and Y376C and move independently of ER structures (Sec61-mCherry). Images were acquired every 1 second for 1 minute. The video plays at 10 frames s-1 and is thus accelerated 10 times.

FGFR2-activating mutations disrupt cell polarity to potentiate migration and invasion in endometrial cancer cell models

Samantha J. Stehbens, Robert J. Ju, Mark N. Adams, Samuel R. Perry, Nikolas K. Haass, David M. Bryant, and Pamela M. Pollock

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.213678