Supplemental Movie 4

Dynamics of eGFP-tagged FGFR wild type and the activating mutant Y376C intracellular vesicles in Sec61-mCherry (ER) expressing Ishikawa cells. Spinning disc confocal microscopy time-lapse sequence cells co-expressing either, top panel: FGFR-WTeGFP (green) and Sec-61-mCherry (magenta) and corresponding grey scale image below or bottom panel: FGFR-Y376C- eGFP (green) and Sec-61-mCherry (magenta) and corresponding grey scale image below. eGFP vesicles are highly dynamic in both WT and Y376C and move independently of ER structures (Sec61-mCherry). Images were acquired every 1 second for 1 minute. The video plays at 10 frames s^-1 and is thus accelerated 10 times.