Supplemental Movie 5

Wound closure dynamics of Ishikawa cells expressing FGFR wildtype or activating mutants. Ishikawa cells stably expressing eGFP alone (empty vector), WT, N550k or Y376C FGFR were transduced with mCherry to create a high contrast images for cell tracking by widefield epifluorescence imaging (IncuCyte ZOOM®). Left four panels: cells stimulated with Heparin (5 μg/ml) (control). Right four panels: cell stimulated with FGF10 (50 μg/ml) in the presence of Heparin (5 μg/ml). Images were acquired every 2 hours for 24 hours. The video plays back at 10 frames s-1 and is thus accelerated 72000 times.

FGFR2-activating mutations disrupt cell polarity to potentiate migration and invasion in endometrial cancer cell models

Samantha J. Stehbens, Robert J. Ju, Mark N. Adams, Samuel R. Perry, Nikolas K. Haass, David M. Bryant, and Pamela M. Pollock

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.213678