Supplemental Movie 7
Dynamics of endometrial acinar morphogenesis and invasive migration in Ishikawa cells expressing FGFR wild-type or activating mutations. Differential Interference Contrast time-lapse images of Ishikawa acini formed from cells stably expressing eGFP alone (empty vector, upper left panels), eGFP-tagged FGFR wild type (left bottom panels), or activating mutants; N550K (upper right panels), Y376C (lower right panels). Acini were grown in growth factor reduced Matrigel™ overlay assay for 3 days prior to stimulation with either heparin alone or heparin plus FGF10 in 2% serum. Images were acquired every hour for 77 hours. The video plays back at 12 frames s-1 and is thus accelerated 43200 times.