Movie 1

Time-lapse images of RPE cells injected with fluorescent labelled β- and γ-actins.
Time lapse images of microinjected β- and γ-actins in RPE cells. β- and γ-actin were labelled with Alexa Fluor 488 and TMR, respectively and injected into human RPE cells. Scale bar =20 μm. Images were acquired every 10 minutes.

Rapid production of pure recombinant actin isoforms in Pichia pastoris

Tomoyuki Hatano, Salvatore Alioto, Emanuele Roscioli, Saravanan Palani, Scott T. Clarke, Anton Kamnev, Juan Ramon Hernandez-Fernaud, Lavanya Sivashanmugam, Bernardo Chapa-y-Lazo, Alexandra M. E. Jones, Robert C. Robinson, Karuna Sampath, Masanori Mishima, Andrew D. McAinsh, Bruce L. Goode, and Mohan K. Balasubramanian

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.213827

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