Movie 1
Migration of WT BMDCs on LECs. CLEC-2+ (LPS-stimulated) WT BMDCs were seeded on a TNFα-stimulated LEC monolayer. After 10 min, non-adherent BMDCs were washed away and time-lapse imaging was performed for 5 min (1 image every 10sec). Cell tracks of live cells were analyzed using the Manual Tracking plugin in Fiji/ImageJ software.
C-type lectin-like receptor 2 (CLEC-2)-dependent dendritic cell migration is controlled by tetraspanin CD37
J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.214551