Movie 1

Chemotactic sGC and PIP3 responses under a cAMP gradient.
Wild-type AX2 cells expressing PH_PKB-GFP (green) and sGC_N-TMR (red) were stimulated with a pipette containing 40 nM cAMP. The position of the pipette (grey) was controlled by hand manipulation. The video was captured every 5 sec and is shown at 10 frame/sec. Time format is “mm:ss”.