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Movie 1

Chemotactic sGC and PIP3 responses under a cAMP gradient.
Wild-type AX2 cells expressing PHPKB-GFP (green) and sGCN-TMR (red) were stimulated with a pipette containing 40 nM cAMP. The position of the pipette (grey) was controlled by hand manipulation. The video was captured every 5 sec and is shown at 10 frame/sec. Time format is “mm:ss”.