Movie S2

DIC time-lapse imaging of stable cell line expressing PP1-NIPP1 after a release from a thymidine-induced G1/S-phase arrest. Imaging was done as detailed for movie S1. The movie shows that some PP1-NIPP1 expressing cells were arrested in interphase. The latter cells often transiently rounded up but then flattened again and did not go through mitosis within the 60h of filming.

Overexpression of PP1–NIPP1 limits the capacity of cells to repair DNA double-strand breaks

Claudia Winkler, Raphael Rouget, Dan Wu, Monique Beullens, Aleyde Van Eynde, and Mathieu Bollen

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.214932