Movie S6

DIC time-lapse imaging of stable cell line expressing PP1-NIPP1 under asynchronous conditions. Imaging was done as detailed for movie S1. The movie shows that some PP1-NIPP1 expressing cells are arrested in interphase. They often transiently rounded up but then flattened again and did not go through mitosis within the 60h of filming.

Overexpression of PP1–NIPP1 limits the capacity of cells to repair DNA double-strand breaks

Claudia Winkler, Raphael Rouget, Dan Wu, Monique Beullens, Aleyde Van Eynde, and Mathieu Bollen

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.214932