ADAP enhances the persistence and movement of TCR-induced SLP-76 microclusters (ADAP KD movie). J14.SY cells were transiently transfected with a dual expression vector encoding an shRNA specific for the 3’UTR of ADAP and a 3xFlag.TRT marker (ADAP KD). TRT protein is shown in the left panel and in red in the merge, SLP-76 in the middle panel and in green in the merge.
Juliana B. Lewis, Frank A. Scangarello, Joanne M. Murphy, Keith P. Eidell, Michelle O. Sodipo, Michael J. Ophir, Ryan Sargeant, Maria-Cristina Seminario, and Stephen C. Bunnell
J Cell Sci
2018. 131:None-None; doi: 10.1242/jcs.215517