Movie S11

ADAP enhances the persistence and movement of TCR-induced SLP-76 microclusters (ADAP KD/AB movie). J14.SY cells were transiently transfected with a dual expression vector encoding an shRNA specific for the 3’UTR of ADAP and a 3xFlag.TRT.ADAP-120 chimera (ADAP KD/AB). ADAP was expressed at near endogenous levels, as confirmed in Figure 2A. ADAP is shown in the left panel and in red in the merge, SLP-76 in the middle panel and in green in the merge.