Movie S12
The overexpressed C-terminal fragment of ADAP antagonizes SLP-76 microcluster persistence and movement via phosphotyrosine-dependent interactions (WT C-term movie). J14.SY cells were transiently transfected with an expression vector encoding a 3xFlag.TRT-tagged chimera of the wild-type C-terminal fragment of ADAP-120. ADAP is shown in the left panel and in red in the merge, SLP-76 in the middle panel and in green in the merge.
ADAP is an upstream regulator that precedes SLP-76 at sites of TCR engagement and stabilizes signaling microclusters
J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.215517