Movie S13

The overexpressed C-terminal fragment of ADAP antagonizes SLP-76 microcluster persistence and movement via phosphotyrosine-dependent interactions. (Y595F C-term movie) J14.SY cells were transiently transfected with an expression vector encoding a 3xFlag.TRT-tagged chimera of the Y595F mutated C-terminal fragment of ADAP-120. ADAP is shown in the left panel and in red in the merge, SLP-76 in the middle panel and in green in the merge.

ADAP is an upstream regulator that precedes SLP-76 at sites of TCR engagement and stabilizes signaling microclusters

Juliana B. Lewis, Frank A. Scangarello, Joanne M. Murphy, Keith P. Eidell, Michelle O. Sodipo, Michael J. Ophir, Ryan Sargeant, Maria-Cristina Seminario, and Stephen C. Bunnell

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.215517