Movie S14

ADAP enters cytoskeletal contact structures prior to the recruitment of SLP-76. J14.SY cells were transiently transfected with ADAP knockdown-addback vectors as in movies S10-11. A representative cell was captured during its first moments of contact with the stimulatory cover slip. ADAP is shown in the left panel and in red in the merge, SLP-76 in the middle panel and in green in the merge. Note that ADAP is enriched in protrusive structures before SLP-76 is recruited into the contact, that SLP-76 microclusters form at the tips of these projections, and that ADAP enters the nascent microclusters concurrently with SLP-76.