Movie S16

The SLP-76 SH2 domain is dispensable for the transient enrichment of ADAP into contact structures (TRT.ADAP with SLP-76.WT.mYFP). J14 cells were transiently co-transfected with 3xFlag.TRT.ADAP-120.WT and SLP-76.WT.mYFP expression vectors. ADAP is shown in the left panel and in red in the merge, mYFP in the middle panel and in green in the merge.

ADAP is an upstream regulator that precedes SLP-76 at sites of TCR engagement and stabilizes signaling microclusters

Juliana B. Lewis, Frank A. Scangarello, Joanne M. Murphy, Keith P. Eidell, Michelle O. Sodipo, Michael J. Ophir, Ryan Sargeant, Maria-Cristina Seminario, and Stephen C. Bunnell

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.215517