Movie S17

The SLP-76 SH2 domain is dispensable for the transient enrichment of ADAP into contact structures (TRT.ADAP with SLP-76.RK.mYFP). J14 cells were transiently co-transfected with 3xFlag.TRT.ADAP-120.WT and SLP-76.RK.mYFP expression vectors. ADAP is shown in the left panel and in red in the merge, SLP-76 in the middle panel and in green in the merge. Note that ADAP is enriched in the lamellipodia formed in the presence of the SH2 domain-mutated SLP-76 chimera. ADAP also forms labile ‘patches’ in the absence of a functional SLP-76 SH2 domain.