Movie S5

The SLP-76 SH2 domain enables microcluster cohesion (RK STD movie #1). SLP-76 deficient Jurkat T cells (J14 cells) were transiently transfected with an expression vector for an SH2 mutant (R448K; RK) SLP-76.YFP chimera of human origin. Magnification is 3x greater than in movies S1-2.

ADAP is an upstream regulator that precedes SLP-76 at sites of TCR engagement and stabilizes signaling microclusters

Juliana B. Lewis, Frank A. Scangarello, Joanne M. Murphy, Keith P. Eidell, Michelle O. Sodipo, Michael J. Ophir, Ryan Sargeant, Maria-Cristina Seminario, and Stephen C. Bunnell

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.215517

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