Movie 1

Primary human monocytes were perfused over TNF activated HUVEC for a 4 min and 40 sec at a density of 1.0 × 10⁶ per mL. Cells are initially captured from flow, after which they spread, before contracting the spread area just prior to TEM. White arrow shows on the rare occasion a CD14-positive monocyte undergoing full-TEM, highlighting the fact that this flow assay had been optimised for capturing cells in mid-TEM. Scale bar = 100 μm.