Movie 1

Primary human monocytes were perfused over TNF activated HUVEC for a 4 min and 40 sec at a density of 1.0 × 106 per mL. Cells are initially captured from flow, after which they spread, before contracting the spread area just prior to TEM. White arrow shows on the rare occasion a CD14-positive monocyte undergoing full-TEM, highlighting the fact that this flow assay had been optimised for capturing cells in mid-TEM. Scale bar = 100 μm.

Sequential binding of ezrin and moesin to L-selectin regulates monocyte protrusive behaviour during transendothelial migration

Angela Rey-Gallardo, Hannah Tomlins, Justin Joachim, Izajur Rahman, Phoebe Kitscha, Karen Frudd, Maddy Parsons, and Aleksandar Ivetic

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.215541