Movie 2

When scd1 is expressed at very low levels, tea1Δ and tea4Δ cells show PORTLI growth. mCherry-Bgs4 distribution and cell morphology in control cells, tea1Δ, and tea4Δ cells, all in a scd1low cdc2-asM17 mCherry-bgs4 genetic background. Bgs4 on the plasma membrane indicates sites of growth. Cells correspond to those shown in Fig. 3B. scd1 expression was repressed by thiamine addition 24 hr prior to start of imaging. Cdc2 kinase activity was inhibited by 3-BrB-PP1 addition 30 min before imaging. Time interval during acquisition, 10 min; total elapsed time, 420 min; time compression at 15 frames per second playback, 9000X.